The mixture was then centrifuged for 10 minutes at rpm. Extracts were weighed Antiviral activity of camelthor s methanolic extract calculating their percentage yield data not shown.
As such, present studies aimed to screen and determine the anti-dengue activity of methanolic extracts of Andrographis paniculata Burm. The solution was filtered and kept at room temperature to evaporate the solvent.
Objectives Considering the healing and anti-bacterial properties of the extract, and also that traditional stock breeders use it to treat FMD lesions, the antiviral effects of the hydro alcoholic extract of A. Capsicum is highly rich in vitamins A, B, C and E and also contains many minerals such as molybdenum, potassium and manganese.
This study was done to evaluate the biologically active substances in extract of camel thorn and their applications in herbal and traditional medicine.
Viral plaque number reduction assay Inhibition of HSV-1 and 2 replications were estimated with plaque reduction assay. The whole aerial body of A. Statistical difference between treatments and positive control was analysed using unpaired t-test at p value less than 0.
Cell viability was obtained using the following equation. Briefly, monolayers of RBK cells were prepared in well plates. For this assay, 0. Cell lines The Huh-7 cell line was offered by Dr. This could be due to the age and geographical locations of the plants as well as the temperature used during extraction [ 21 ].
Although methanolic extracts of O. Table 1 The total flavonoids content of six medicinal plants as determined through aluminium chloride colourimetric assay. Orientin and vicenin are reported to have radical scavenging activity [ 16 ] while lutoelin is known to be anti-inflammatory agent and have therapeutic action against multiple sclerosis [ 1718 ].
In Iran, FMD is the most serious cattle disease and the most prominent minatory factor for dairy industrials. Some botanical constituents such as glycyrrhizin, catechin, silymarin, phytosterols, the antioxidants N-acetylcysteine and vitamin E were assessed for their efficiency in curing chronic hepatitis and liver damage [ 19 ].
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4. The development of a dengue vaccine is complicated by the antibody-dependent enhancement effect.
The medium was subsequently discarded from the wells, and the cells were washed with clean medium twice and covered again with a fresh medium containing 1. In this study, A. A blank control medium only and cell control cells only were also plated. Thus, the dengue virus obtained was confirmed to be of serotype 1 DENV A primary infection with any of the four serotypes results in a lifelong immunity to that serotype, and temporary immunity to the others.
Materials and Methods 3. After 24 h solvents were filtered, residue was soaked again in methanol. It has small, pink to red pea flowers, and brown legume pods 5.
The medium was then filter sterilised using 0. Extraction of Medicinal plants Thirteen different plants were collected and dried on the basis of their medicinal characteristics.
The solution was then mixed with 0. The cytotoxicity assay was initiated by seeding 1. This activity may justify its use as a source of natural compounds to control FMDV in infected animals. Table 1 List of selected Pakistani Medicinal plants.
Natural products could be enormously employed as sources for anti-HCV mediator [ 21 ].
The supernatant of the cell culture was removed as a source of the virus. HCV belongs to the family Flaviviridae which is enveloped viruses harboring positive-sense single-stranded RNA genome [ 45 ]. The viral cytopathic effect CPE was examined daily using a light microscope. These indigenous plants were collected from different climatic zones of Pakistan.
Background Dengue disease regardless of its serotypes is transmitted from person to person by Aedes aegypti and Aedes albopictus mosquitoes in the domestic environment [ 1 ].
A similar protocol was used for viral inoculation as established by Zekari et al. Vaccination or infection against one serotype does not cross-protect against other serotypes; therefore, constant surveillance and testing potential cures is essential 3. Cytotoxicity of the extract was measured by an MTT assay.extract of A.
digitata has shown significant antiviral activity against Herpes simplex, Sindibis and Polio virus (Anani et al., ). Antibacterial and anti-Trypanosome activities have also been indicated by the plant extracts Antibacterial, Antifungal and Antitubercular activity of methanolic extracts.
Evaluation of antiviral and cytotoxic activities of methanolic extract of S. grandiflora (Fabaceae) flowers Saravana Kumar Arthanari 1*, Jayachandran Vanitha 2, Mani Ganesh 3, Krishnasamy Venkateshwaran 4, De Clercq 5.
The aim of this study is to evaluate the antiviral and cytotoxic activities of methanolic extract of S. grandiflora flowers growing in southern parts of Tamilnadu, India.
2. Materials and methods Antiviral activity of crude extracts of Eugenia jambolana Lam. against highly pathogenic avian influenza (H5N1) virus Richa Sood a, *, D Swarup b, S Bhatia a, D D Kulkarni a, S Dey b, M Saini b & S C Dubey a.
This study was conducted to quantify the antioxidant and anti-viral activities of Capsicum annuum methanolic extracts. The extract of Capsicum annuum was prepared by.
The methanolic extract of Verbascum thapsus was evaluated for its antiviral activity against the pseudorabies virus strain RC/79 (PrV), and also for its cytotoxic activity on Vero cells. The extract showed CC 50 values of µg mL −1 and µg mL −1 by NRU and MTT assays, respectively.Download